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pAAV-Syn-DIO-PinkyCaMP

Plasmid

#232857

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

Depositor

Olivia Masseck

Publication

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

u6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-FADD-DD

Plasmid

#58263

Purpose

Lentiviral vector for expressing a truncated form of FADD (lack of death effector domain) in mammalian cells

Depositor

Joan Massague

Publication

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

FADD (FADD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

version of FADD (FADD-DD) that contains the FADD …

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pF142 pAcGFP1 SOD1 A4V

Plasmid

#26403

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDK1

Plasmid

#23804

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK1 (PDK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pIVM_02

Plasmid

#204500

Purpose

Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

Depositor

Alessio Ciulli

Publication

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

amino acids 54-213 VHL (VHL Human)

Tags

6xHis, TEV, and Thrombin

Expression

Bacterial

Mutation

0

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRE_tau-LacZ::tTA-H100Y

Plasmid

#34927

Depositor

Mark Mayford

Publication

Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Inserts

tau (MAPT Bovine)
tTA

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Mutation

Made doxycycline insensitive by introduction of a…

Promoter

tetO

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR4_G3BP1

Plasmid

#127104

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Use

Gateway Cloning

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-EPHB1

Plasmid

#23930

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB1 (EPHB1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-PTK7

Plasmid

#23943

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK7 (PTK7 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-LIMK1

Plasmid

#23511

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LIMK1 (LIMK1 Human)

Use

Gateway Cloning

Mutation

L273P

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGreen_GUS_competitor

Plasmid

#55208

Purpose

GUS-based microRNA target competitor used for testing function as target mimicry

Depositor

Michael Axtell

Publication

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Insert

beta-glucuronidase

Use

Plant expression

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEvol-pAcFRS.1.t1

Plasmid

#73545

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.1.t1
pAcFRS.1.t1

Expression

Bacterial

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Tn7 integration plasmid

Plasmid

#154134

Purpose

Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

Depositor

Shimon Bershtein

Publication

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Inserts

left and right end of Tn7 arm
Spectinomycin resistant gene

Expression

Bacterial

Promoter

Spectinomycin resistance gene promoter and no

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Publication

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Publication

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pEF6-myc-TBC1D15

Plasmid

#79148

Purpose

mammalian expression of TBC1D15

Depositor

Aimee Edinger

Publication

Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

Insert

TBC1D15 (Tbc1d15 Mouse)

Tags

His, Myc, and V5

Expression

Mammalian

Mutation

S616N (please see depositor comment below)

Promoter

EF-1alpha

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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