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macroH2A1.1-F192V-GFP (pc5114)

Plasmid

#223437

Purpose

Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-F192V (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 P652_E653 del

Plasmid

#213700

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

Depositor

Veerle Baekelandt

Article

Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

Insert

human ATP13A2 P652_E653 del (ATP13A2 Human)

Use

Lentiviral

Mutation

WT, D962N

Promoter

CMV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-OFF-ArgiNLS-AausFP1

Plasmid

#220605

Purpose

Flp-independent expression of a single-cell discriminating version of AausFP1 fluorescent protein. Flp activity can turn off expression.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A8_His

Plasmid

#201718

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A8 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A10-5_His

Plasmid

#201720

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A10-5 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_B5-5_His

Plasmid

#201721

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH B5-5 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-N57G

Plasmid

#193651

Purpose

Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

Plasmid

#201576

Purpose

Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutations S172A, M173A and V195A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Stx5L(m55V)-GFP

Plasmid

#209845

Purpose

Expresses Stx5 M55V mutant fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Methionine 55 to Valine, causes loss of S…

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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B-HypaR-SpCas9

Plasmid

#126764

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A; N692A; M694A; Q695A; H698A; amino acids 10…

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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