We narrowed to 3,058 results for: bid

1. pCIB1(deltaNLS)-pmGFP

   Plasmid

   #28240

   Purpose

   Expresses CIB1 fused to plasma membrane-localized EGFP

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL175

   Plasmid

   #22276

   Purpose

   Expresses YFP-PIF for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSTARR-seq_SCP1 vector

   Plasmid

   #99292

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTpal-fdc

   Plasmid

   #78288

   Purpose

   Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

   Depositor

   David Nielsen

   Article

   McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

   Inserts

   PAL2 (PAL2 Mustard Weed)
   FDC1 (FDC1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   *see below

   Promoter

   trc

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLP700

   Plasmid

   #203742

   Purpose

   C-terminal tagging of target proteins with AID*/3xHA degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain codons 71-114
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHLP701

   Plasmid

   #203743

   Purpose

   C-terminal tagging of target proteins with 3xV5-IAA17 degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xV5

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. B2375_CRY2olig-mEGFP

   Plasmid

   #215109

   Purpose

   Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CRY2olig-mEGFP (CRY2 Mustard Weed)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   E490G, 1-498

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE666

    Plasmid

    #153231

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1hSIRT4_HA

    Plasmid

    #24482

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 4 (SIRT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cry2L348F(1-531)-mCh-BAXS184E

    Plasmid

    #117239

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531.L348F-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM Mustard Weed, A. tumefaciens)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMALB

    Plasmid

    #161784

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE479

    Plasmid

    #153270

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myristoylated optoDroplet

    Plasmid

    #111507

    Purpose

    For membrane localized optoDroplets

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    Cry2 (CRY2 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    FUS, Fusion Red, and Myristoylation Sequence (MYR…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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