We narrowed to 4,802 results for: pcr

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p164 Dync1i1.B

Plasmid

#26435

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pCZGY2254

Plasmid

#101247

Purpose

GFP subunits 1-10, (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp1-10

Use

Gateway Cloning

Tags

GFP1-10

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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CCR2-DuET

Plasmid

#213198

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR2 (CCR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs15

Plasmid

#109360

Purpose

GsX chimera with Gα15 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs15 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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ML12

Bacterial Strain

#61910

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE avtA aspC genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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NbV5-HiBiT

Plasmid

#201489

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HiBiT

Tags

HiBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP2-NbALFA

Plasmid

#201486

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbALFA

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pACT5FVMF(f)

Plasmid

#110549

Purpose

Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

Depositor

David Russell

Article

Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

Insert

EGFP, FV LTRs

Use

Qpcr control for titering foamy virus

Promoter

promoter: MLV LTR

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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DH5alpha::DddI

Bacterial Strain

#179631

Purpose

For expression of toxic DddA

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Bacterial Resistance

Gentamicin

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only
TB205 △trpC

Bacterial Strain

#230038

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC

Bacterial Strain

#230037

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC

Bacterial Strain

#230036

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △proC

Bacterial Strain

#230035

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
OPRM1-DuET

Plasmid

#213361

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRM1 (OPRM1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-z ONE-GO

Plasmid

#189734

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-z ONE-GO
GNAZ (Gnaz Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-IRESWT-Gbetagamma ONE-GO

Plasmid

#204339

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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GPR17-Tango

Plasmid

#66336

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR17 (GPR17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only