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pGGB-V5-TurboID-T2A-C

Plasmid

#218896

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-T2A

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB-Kozak-ATG-C

Plasmid

#218895

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

kozak-ATG

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-W-ccdB-Z

Plasmid

#218893

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-A-ccdB-G-Z

Plasmid

#218892

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN-W-A-ccdB-G-X

Plasmid

#218891

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN-X-hPGK-PuroR-Z

Plasmid

#218904

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-PuroR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-NeoR-SV40 polyA-Z

Plasmid

#218909

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-Neomycine-SV40 polyA

Expression

Mammalian

Mutation

395T>C

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-PuroR-SV40 polyA-Z

Plasmid

#218908

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-PuroR-SV40 polyA

Expression

Mammalian

Mutation

297T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-BlastR-Z

Plasmid

#218907

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-BlastR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-NeoR-Z

Plasmid

#218906

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-NeoR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-HygroR-Z

Plasmid

#218905

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-HygroR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN-X-mPGK-BlastR-SV40 polyA-Z

Plasmid

#218903

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-BlastR-SV40 polyA

Expression

Mammalian

Mutation

2468T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3A

Plasmid

#35521

Purpose

Mammalian expression of human DNMT3A with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3A (DNMT3A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B1

Plasmid

#35522

Purpose

Mammalian expression of human DNMT3B1 with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B1 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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PT2/C-Luc//PGK-SB13

Plasmid

#20207

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

Flirefly luciferase/Sb transposase

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc TCF4

Plasmid

#16512

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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GRK2-K220R

Plasmid

#35403

Depositor

Robert Lefkowitz

Article

Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Mutation

K220R

Promoter

CMV

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-MBD1

Plasmid

#47057

Purpose

Expresses methyl-CpG binding domain protein 1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Domain Protein 1 (MBD1 Human)

Use

Gateway entry vector

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA1-GATA6

Plasmid

#51929

Purpose

mammalian expression of mouse GATA6

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA6 (Gata6 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PpipB-GFPLVA

Plasmid

#23344

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPMA3dPlac-PprgH-GFPLVA

Plasmid

#23340

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-null-GFPLVA

Plasmid

#23338

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pT/CMV-SV40-LgT

Plasmid

#20204

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

SV40LgT

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E IRES GFP

Plasmid

#18761

Depositor

Scott Lowe

Article

Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

Insert

eIF4E (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
PT2/C-fluc

Plasmid

#20203

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

C-Fluc

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MAP4-C-10

Plasmid

#54152

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MAP4

Tags

mEmerald

Expression

Mammalian

Mutation

aa 640-1045 of MAP4

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pENTR-FOXP2

Plasmid

#47053

Purpose

Expresses forkhead box P2

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Forkhead Box P2 (FOXP2 Human)

Use

Gateway entry vector

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-A1Up

Plasmid

#16170

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Ubiquilin 4 (UBQLN4 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pLEG(R1-R3) #BGV149

Plasmid

#48956

Purpose

Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PssaG-GFPLVA

Plasmid

#23343

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pHR TCF4

Plasmid

#16514

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS (A) Wnt3a (CT#169)

Plasmid

#13976

Depositor

Cliff Tabin

Article

Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

Insert

Wnt3a (Wnt3a Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-HOXA1

Plasmid

#47054

Purpose

Homeobox A1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Homeobox A1 (HOXA1 Human)

Use

Gateway entry vector

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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