We narrowed to 21,180 results for: ATO

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Article

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mDrc7-FLAG

Plasmid

#176470

Purpose

Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

dynein regulatory complex subunit 7 (Drc7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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his3MX6-ins3

Plasmid

#195040

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

His5+

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36881

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Neurog2

Plasmid

#99695

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)

Use

AAV and CRISPR

Tags

VP64

Mutation

dead Cas9

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

Plasmid

#218558

Purpose

Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-MLLx3-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

Plasmid

#225490

Purpose

Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Neuronal expression

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

Plasmid

#224779

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Plasmid

#224755

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C/C159A/C219A/C365A/C399A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Plasmid

#224756

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A/C159A/C219A/C365A/C399A

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB Ubiqutin-BFP

Plasmid

#202426

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

Ubiquitin, TagBFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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