We narrowed to 13,843 results for: Cas9

1. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   pU6 5'CMV and EF1-alpha

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH231-EF1-BLST-dCas9-VPR

   Plasmid

   #115148

   Purpose

   Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   BlastR-P2A-dCas9-VPR
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SauriCas9-KKH-Puro

   Plasmid

   #135966

   Purpose

   Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

   Plasmid

   #221551

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE3G-VP64-dCas9-VP64-ZsG

   Plasmid

   #192668

   Purpose

   Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   VP64-dCas9-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   TRE3G

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

   Plasmid

   #46168

   Purpose

   Codon optimized Cas9_SV40 NLS with intron

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   codon optimized Cas9_SV40 NLS with intron
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   intron inserted at position 3113-3163, and SV40 N…

   Promoter

   eef-1A.1 (eft-3)

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST1374-NLS-flag-linker-Cas9

   Plasmid

   #44758

   Depositor

   Xingxu Huang

   Article

   Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

   Insert

   Human codon optimized Cas9
   

   Use

   CRISPR

   Tags

   32 amino acid linker, Flag, and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. p300 core-dCas9-CBP core

   Plasmid

   #179559

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-nls-st1dCas9-3nls-3XGFP

    Plasmid

    #64111

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

    Plasmid

    #160626

    Purpose

    TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-dCas9:TV-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

    Plasmid

    #165082

    Purpose

    gRNA 1 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid

    #172293

    Purpose

    In vitro transcription of Cas9-T2A-mClover3 synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    Cas9-T2A-mClover3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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