We narrowed to 2,477 results for: T2A

1. RFP-KIF1B beta-FL

   Plasmid

   #79111

   Purpose

   Mammalian expression of RFP-KIF1B beta-FL

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   RFP-KIF1B beta full length (KIF1B Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pT22UbhaEng2aE4PCh(#271)

   Plasmid

   #184085

   Purpose

   cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-ZfEng2a-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT22UbhaEng2bE4PCh(#272)

   Plasmid

   #184086

   Purpose

   cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-ZfEng2b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT22gCfpUbm5En2E4PCh(#251)

   Plasmid

   #184083

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT22Ubm5Eng2bE4PCh(#284)

   Plasmid

   #184088

   Purpose

   cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT22UbHEn2E4PCh(#262)

   Plasmid

   #184084

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT22Ubm5Eng2aE4PCh(#283)

   Plasmid

   #184087

   Purpose

   cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2a-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-KIF1B beta-600-1400

   Plasmid

   #79109

   Purpose

   Mammalian expression of GFP-KIF1B beta-600-1400

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pT2iApowriter2(#336)

   Plasmid

   #184104

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   internal tag: 5myc

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT22Ubm5En2SRE4PCh(#302)

    Plasmid

    #184089

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2(SR)-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYJA5

    Plasmid

    #217778

    Purpose

    The empty vector for quadruple sgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Puromycin resistance gene, T2A, and TagBFP

    Expression

    Mammalian

    Promoter

    PGK, CMV, U6

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-CuO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-TR-hSOD1

    Plasmid

    #232478

    Purpose

    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-OSKM

    Plasmid

    #20328

    Purpose

    Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-tetO-hNIL

    Plasmid

    #197089

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge