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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 transition and G2/M transition, one of two plasmids for the FUCCI4 system

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

Insert

desmoglein-3 (Dsg3 Mouse)

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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AID-BE3

Plasmid

#100803

Purpose

Expresses AID-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

activation induced cytidine deaminase (AICDA Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2-msfGFP

Plasmid

#180318

Purpose

mammalian expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CHFR-PBZ-eGFP

Plasmid

#211581

Purpose

Expressing CHFR PBZ domain fused to eGFP

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

CHFR PBZ domain (CHFR Human)

Tags

eGFP

Expression

Mammalian

Mutation

PBZ domain of the protein

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_mApple-SEPT7 Gmut2

Plasmid

#180317

Purpose

mammalian expression of human SEPT7 Gmut2 fused to mApple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

Plasmid

#111502

Purpose

Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].

Depositor

Michael Lin

Article

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

ZF(VEGFA)-StaPL(AI)-YFP-VPR

Expression

Mammalian

Mutation

The HCV NS3 protease carries V36M, T54A, and S122…

Promoter

CMV

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 N C-V5-tag

Plasmid

#179987

Purpose

Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 N

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP10-14-SEPT7

Plasmid

#180341

Purpose

mammalian expression of human SEPT7 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pLEX307_PDHA2S293A

Plasmid

#115197

Purpose

Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75120

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP10

Plasmid

#180343

Purpose

mammalian expression of human SEPT9_i1 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 M C-V5-tag

Plasmid

#179986

Purpose

Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 M

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

Plasmid

#75121

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRG1

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only