We narrowed to 31,312 results for: PLE;
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Plasmid#115218PurposeExpresses PguCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPguCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Barcode backbone
Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE2
Plasmid#195368PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTENDepositorInserthA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…ExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
TagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ago1
Plasmid#21533DepositorAvailable SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)1
Plasmid#126954PurposeBacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagseGFPExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PV1_VP1
Plasmid#202071PurposeBacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertPV1 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)
Plasmid#184394PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globinDepositorInsertRenilla luciferase
Tags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-NavMs
Plasmid#100004PurposeElectrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFPDepositorInsertHis-NavMs, Voltage gated sodium channel
TagsHexa-His tag followed by a thrombin cleavage siteExpressionMammalianMutationC-terminus from a codon-optimized synthetic gene,…PromoterCMVAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only