We narrowed to 43,144 results for: cha

1. pEXP5-NT/pT7 LuxI

   Plasmid

   #193626

   Purpose

   Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEXP5-NT/pT7 LuxR

   Plasmid

   #193625

   Purpose

   Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH-tdGFP

   Plasmid

   #74322

   Purpose

   pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

   Depositor

   Joerg Stelling

   Article

   Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

   Insert

   pH-tdGFP
   

   Use

   Yeast tagging

   Tags

   none

   Expression

   Yeast

   Mutation

   N149Y, Q204H

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEXP5-NT/pT7 LasR

   Plasmid

   #193629

   Purpose

   Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LasR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YEp195-CUP1-His-Ubi

   Plasmid

   #99540

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS423-CUP1-His-Ubi

   Plasmid

   #99541

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YEp181-CUP1-His-Ubi

   Plasmid

   #99539

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-oChIEF-tdTomato

   Plasmid

   #50977

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426-GAL-Alpha-synuclein-E46K

   Plasmid

   #181723

   Purpose

   Galactose inducible expression of Alpha-synuclein-E46K

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   E46K

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYN4+

    Plasmid

    #78715

    Purpose

    mammalian expression of NHE1

    Depositor

    Larry Fliegel

    Article

    Reddy et al J Biol Chem. 2008 Aug 8;283(32):22018-30

    Insert

    NHE1 (SLC9A1 Human)
    

    Use

    Tags

    HA3x

    Expression

    Mammalian

    Mutation

    D720G

    Promoter

    RSV-LTR

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRP6-pCS2-VSVG

    Plasmid

    #27282

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB19-PapRS#34-OmpA

    Plasmid

    #231105

    Purpose

    Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

    Depositor

    Arne Skerra

    Article

    Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H50Q

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAMS366-4xCDRE-GFP-PEST

    Plasmid

    #138658

    Purpose

    destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

    Depositor

    Sabrina Büttner

    Article

    Diessl et al Microbial Cell, 2020: in press

    Insert

    4xCDRE-CYC1(PM)-yEGFP3-PEST
    

    Use

    Yeast reporter plasmid

    Tags

    Expression

    Mutation

    Promoter

    CYC1

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CF-PIPKi

    Plasmid

    #20158

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D253A mutation in PIPK. C-terminus (amino acids …

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH3FS

    Plasmid

    #85780

    Purpose

    N-ICE plasmid pH3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG426-GAL-Alpha-synuclein-A53T

    Plasmid

    #181722

    Purpose

    Galactose inducible expression of Alpha-synuclein-A53T

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A53T

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MXS_CMV

    Plasmid

    #62417

    Purpose

    MXS_chaining vector with CMV

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MXS_P2A

    Plasmid

    #62433

    Purpose

    MXS_chaining vector with P2A

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Self-cleaving peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MXS_bGpA

    Plasmid

    #62424

    Purpose

    MXS_chaining vector with bGpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    beta-Globin poly A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CF-PIPK

    Plasmid

    #20157

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-PIPK (Pip5k1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminus (amino acids 636-661)truncated, R445E,…

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBACgus N-His/GST/LRRK2 1867-2186

    Plasmid

    #40403

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNRB150

    Plasmid

    #36451

    Depositor

    Douglas Bishop

    Article

    Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

    Insert

    DMC1 (DMC1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MXS_E2-Crimson

    Plasmid

    #62410

    Purpose

    MXS_chaining vector with E2-Crimson

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YIp128-P30-His-POL30

    Plasmid

    #99542

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG416-GAL-FUS

    Plasmid

    #181711

    Purpose

    Galactose inducible expression of FUS

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p3A9cbetaTCR

    Plasmid

    #69600

    Purpose

    TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pIM1708-surT-PsurP-agaN-pNW33N

    Plasmid

    #44009

    Depositor

    Ichiro Matsumura

    Article

    Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

    Insert

    surT-PsurP-agaN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted EcoRI site (synonymous change, C to T at …

    Promoter

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p413TEF-Sir2-H364Y

    Plasmid

    #51743

    Purpose

    overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H364Y

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pK3FS

    Plasmid

    #85776

    Purpose

    N-ICE plasmid pK3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p404-Grx5-GFP1-10

    Plasmid

    #91952

    Purpose

    Expresses GFP1-10 in the mitochondrial matrix

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    Grx5-GFP1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pH3F-GAL1

    Plasmid

    #85775

    Purpose

    N-ICE plasmid pH3F-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. UBC13-PGEX4T

    Plasmid

    #25464

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    Ubc13 (UBC13, MMS2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MXS_BiTet

    Plasmid

    #62423

    Purpose

    MXS_chaining vector with BiTet

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Bidirectional tetracycline inducible promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge