We narrowed to 7,241 results for: 11

1. Pdgfra-T2A-H2B-eGFP donor

   Plasmid

   #113120

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPLtetO-ClpX

   Plasmid

   #89657

   Purpose

   Plasmid for titrating synthesis of ClpX with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   clpX
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ906

   Plasmid

   #44472

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, [4-3] Gateway
   

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

5. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

   Plasmid

   #183069

   Purpose

   Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   CRISPR

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-INU-TEX264 LIR4A-FLAG

   Plasmid

   #128259

   Purpose

   Expresses TEX264 LIR4A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   TEX264 LIR4A (TEX264 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed FEEL (aa 273-276) to alanines

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBD-ESCRT 0

   Plasmid

   #21499

   Depositor

   James Hurley

   Article

   Ren et al Structure. 2009 Mar 11. 17(3):406-16.

   Insert

   pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
   

   Use

   Tags

   GST and His-MBP

   Expression

   Insect

   Mutation

   none

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pMSP2

   Plasmid

   #26282

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP2
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of dimer of deletion mutant (1-43)…

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pNAS1B split mCherry N-link/link-C

    Plasmid

    #61965

    Purpose

    Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-link
    link-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSC236

    Plasmid

    #216368

    Purpose

    xhNup155-Nb2i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb2i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSC235

    Plasmid

    #216372

    Purpose

    xhNup155-Nb3i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb3i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSC227

    Plasmid

    #216351

    Purpose

    xhNup35-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. shLuc pRRL

    Plasmid

    #19125

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIntegrase_5

    Plasmid

    #60577

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5 mFz8CRD-IgG

    Plasmid

    #16689

    Depositor

    Xi He

    Article

    Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

    Insert

    Frizzled-8 (Fzd8 Mouse)
    

    Use

    Tags

    IgG

    Expression

    Mammalian

    Mutation

    CRD domain of mFz8

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS_FLPER(T2)

    Plasmid

    #31303

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    full length FLP linked to ligand binding domain o…

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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