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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,425 results for: ef1

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Showing: 2281 - 2300 of 9425 results

  2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  4. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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  5. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  8. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  13. AAV-EF1α-DIO-DSE-mCherry-PSE-shRNA-Scramble

    Plasmid
    #250237
    Purpose
    Cre-dependent AAV expressing mCherry and a scrambled shRNA under EF1α promoter. Used as a non-targeting control for shRNA knockdown
    Depositor
    Won Do Heo
    Article
    Shin et al Exp Mol Med. 2025 Aug;57(8):1818-1836. d
    Insert
    shRNA-Scramble (SMARCA4 Synthetic)
    Use
    AAV, Cre/Lox, Mouse Targeting, RNAi, and Syntheti…
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    250237-plasmid-map-sequence-id-497741

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Showing: 2281 - 2300 of 9425 results