We narrowed to 3,645 results for: MAK;

1. pSaCas9-1xms2-2x3’UTR

   Plasmid

   #122946

   Purpose

   For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   SaCas9-MS2-HBB 3' UTR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-XL4 ASXL1 (1-1304)

   Plasmid

   #74244

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-1304

   Promoter

   CMV

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

   Plasmid

   #35500

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. K785R Smarca4-V5 IRES-puro

   Plasmid

   #107059

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Luciferase

   Plasmid

   #213979

   Purpose

   Expresses Doxy Inducible Luciferase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   6xHis affinity tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

   Plasmid

   #35497

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn Coff/FonEYFP

   Plasmid

   #55652

   Purpose

   Cre-off/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV; Cre off/flp on eyfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-CMV-XRCC1-EGFP-Hygro

    Plasmid

    #176062

    Purpose

    EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

    Plasmid

    #35504

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

    Plasmid

    #74262

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. BAP1

    Plasmid

    #81024

    Purpose

    Retroviral vector containing human BAP1 and Thy1.1 selection marker

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

    Plasmid

    #35502

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-TetO-Cdx2

    Plasmid

    #128829

    Purpose

    dox-dependent expression of mouse Cdx2

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn Con/Foff EYFP

    Plasmid

    #55651

    Purpose

    Cre-on/Flp-off EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp off eyfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid

    #35509

    Purpose

    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV5 and AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCX0393

    Plasmid

    #205984

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTarget: Ptaq-RFP_PlacIq-GFP

    Plasmid

    #192280

    Purpose

    RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Inserts

    RFP
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacIq and Ptaq

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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