We narrowed to 6,138 results for: WUS;

1. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

   Plasmid

   #170229

   Purpose

   Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR3-FLAG-TRAF6-C70A

   Plasmid

   #43926

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   TNF Receptor-Associated Factor 6 (Traf6 Mouse)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   C70A

   Promoter

   Available since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-kanMX6-P41nmt1

   Plasmid

   #39281

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pFA6a-kanMX6-P81nmt1

   Plasmid

   #39282

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2 NT sgRNA3

   Plasmid

   #164929

   Purpose

   Expresses Non-targeting sgRNA3 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA3 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX305-N-dTAG-CARD8

   Plasmid

   #169989

   Purpose

   Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   dTAG

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK4_002

   Plasmid

   #123811

   Purpose

   Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   SV40 pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. dTAG NLRP1

   Plasmid

   #166825

   Purpose

   dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   IRFP and LEXY

   Expression

   Insect

   Mutation

   Promoter

   maternal tubulin

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-DIO-mNeonGreen

    Plasmid

    #99132

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-hSyn-Galpha-q ONE-GO

    Plasmid

    #204336

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-q ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK4_003

    Plasmid

    #123812

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-tdTomato

    Plasmid

    #99116

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti_MS2_P65_HSF1_2A_puro

    Plasmid

    #118699

    Purpose

    To express MS2-P65-HSF1

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-GFAP-mKate2.5f

    Plasmid

    #99129

    Purpose

    Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mKate2.5f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dnase1L3 R206C pIRES-DsRed

    Plasmid

    #98847

    Purpose

    Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Dnase1L3 (DNASE1L3 Human)
    

    Use

    Tags

    IRES DsRed

    Expression

    Mammalian

    Mutation

    R206C which greatly reduces nuclease activity

    Promoter

    CMV

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac_human-pro-IL-18

    Plasmid

    #214337

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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