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We narrowed to 2,424 results for: dcas9

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Showing: 2321 - 2340 of 2424 results

  1. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  2. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Publication
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  3. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  5. pCMV-dCas13-NES-eNAT10

    Plasmid
    #238299
    Purpose
    Expresses cytoplasmic version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Publication
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    HIV NES (C terminal of dPspCas13b)
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238299-plasmid-map-sequence-id-472414

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  6. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194885
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Publication
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194885-plasmid-map-sequence-id-382817

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  9. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Publication
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Promoter
    TRE3GV
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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  10. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  11. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109430-plasmid-map-sequence-id-212945

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  12. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  15. pSJ105

    Plasmid
    #208859
    Purpose
    Expresses SoxS activation domain and gRNA targeting 105 region within the synthetic promoter in bacterial cell
    Depositor
    Marc Erhardt
    Publication
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    sgRNA 105
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    CRISPR/dCas9-responsive synthetic promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208859-plasmid-map-sequence-id-415447

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  16. pSJ107-2x

    Plasmid
    #208812
    Purpose
    Expresses SoxS activation domainand gRNA targeting two copies of 107 region within the synthetic promoter in bacterial cell
    Depositor
    Marc Erhardt
    Publication
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    sgRNA 107-2x_mRFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    CRISPR/dCas9-responsive synthetic promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208812-plasmid-map-sequence-id-418905

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  17. pSJ106-2x

    Plasmid
    #208860
    Purpose
    Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell
    Depositor
    Marc Erhardt
    Publication
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    sgRNA 106-2x_mRFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    CRISPR/dCas9-responsive synthetic promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208860-plasmid-map-sequence-id-415439

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  19. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid
    #238300
    Purpose
    Expresses nuclear version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Publication
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238300-plasmid-map-sequence-id-474324

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  20. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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Showing: 2321 - 2340 of 2424 results