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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,069 results for: grn

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Showing: 2321 - 2340 of 23069 results

  1. pX330 Ctnnb1.1

    Plasmid
    #59911
    Purpose
    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    59911-plasmid-map-sequence-id-123316

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  6. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Publication
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    182746-plasmid-map-sequence-id-358079

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  8. pMOD_B2515

    Plasmid
    #91072
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    91072-plasmid-map-sequence-id-181899

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  14. pU6T7

    Plasmid
    #71462
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)
    Depositor
    Willie Mark
    Publication
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    71462-plasmid-map-sequence-id-128762

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  15. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid
    #103867
    Purpose
    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    W85X targeting guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103867-plasmid-map-sequence-id-199148

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  18. LentiRCas9-CUG

    Plasmid
    #104183
    Purpose
    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Publication
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-CUGsgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104183-plasmid-map-sequence-id-201743

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  19. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  20. pU6_HEK3_nsgRNA(PP7)

    Plasmid
    #232444
    Purpose
    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232444-plasmid-map-sequence-id-462757

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Showing: 2321 - 2340 of 23069 results