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AMIGO-1 scFv [L86/33]

Plasmid

#206746

Purpose

Mammalian Expression of AMIGO-1 scFV. Derived from hybridoma L86/33 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

AMIGO-1 (Rattus norvegicus) recombinant scFV (Amigo1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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SynDIG3/Tmem91 scFv [N160/21]

Plasmid

#206748

Purpose

Mammalian Expression of SynDIG3/Tmem91 scFV. Derived from hybridoma N160/21 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SynDIG3/Tmem91 (Rattus norvegicus) recombinant scFV (Tmem91 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kv1.7 K+ channel scFv [N471/27]

Plasmid

#206753

Purpose

Mammalian Expression of Kv1.7 K+ channel scFV. Derived from hybridoma N471/27 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.7 K+ channel (Mus musculus) recombinant scFV (Kcna7 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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VSP scFv [N432/21]

Plasmid

#206756

Purpose

Mammalian Expression of VSP scFV. Derived from hybridoma N432/21 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VSP (Rattus norvegicus) recombinant scFV (tpte Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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VSP scFv [N432/63]

Plasmid

#206757

Purpose

Mammalian Expression of VSP scFV. Derived from hybridoma N432/63 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VSP (Rattus norvegicus) recombinant scFV (tpte Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AMIGO-1 scFv [L86/14]

Plasmid

#206767

Purpose

Mammalian Expression of AMIGO-1 scFV. Derived from hybridoma L86/14 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

AMIGO-1 (Mus musculus) recombinant scFV (Amigo1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AMIGO-1 scFv [L86/2]

Plasmid

#206768

Purpose

Mammalian Expression of AMIGO-1 scFV. Derived from hybridoma L86/2 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

AMIGO-1 (Mus musculus) recombinant scFV (Amigo1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Tiam1 scFv [L130/1]

Plasmid

#190510

Purpose

Mammalian Expression of Tiam1 scFV. Derived from hybridoma L130/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Tiam1 (Mus musculus) recombinant scFV (Tiam1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Kv1.3 K+ channel scFv [L23/27]

Plasmid

#190514

Purpose

Mammalian Expression of Kv1.3 K+ channel scFV. Derived from hybridoma L23/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.3 K+ channel (Rattus norvegicus) recombinant scFV (Kcna3 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Shank1 scFv [N22/21]

Plasmid

#190539

Purpose

Mammalian Expression of Shank1 scFV. Derived from hybridoma N22/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank1 (Rattus norvegicus) recombinant scFV (Shank1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Shank2 scFv [N23B/6]

Plasmid

#190543

Purpose

Mammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Alpha4 scFv [N398A/34]

Plasmid

#190551

Purpose

Mammalian Expression of GABA(A)R, Alpha4 scFV. Derived from hybridoma N398A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Alpha4 (Rattus norvegicus) recombinant scFV (Gabra4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Alpha2 scFv [N399/19]

Plasmid

#190552

Purpose

Mammalian Expression of GABA(A)R, Alpha2 scFV. Derived from hybridoma N399/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Alpha2 (Rattus norvegicus) recombinant scFV (Gabra2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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RGS14 scFv [N133/21]

Plasmid

#182081

Purpose

Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/81]

Plasmid

#182073

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-ccDockerin

Plasmid

#72200

Purpose

MBP-ccDockerin expression plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

Insert

Clostridium Cellulolyticum Type I Dockerin

Tags

His-Tag and Maltose Binding Protein

Expression

Bacterial

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc C1A4E

Plasmid

#141088

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL1-2

Plasmid

#109006

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-sgRNA #2 (ATL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETC PITPbeta N89F

Plasmid

#58279

Purpose

bacterial expression of PITP beta N89F mutant

Depositor

Shamshad Cockcroft

Article

Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

N89F (abolishes PI binding)

Promoter

T7

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only