We narrowed to 2,383 results for: mia

1. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Use

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-iPE-Plus

   Plasmid

   #214062

   Purpose

   inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207863

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEL1200

   Plasmid

   #138526

   Purpose

   For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

   Insert

   mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)
   

   Use

   Tags

   N-terminal His tag and TEV cleavage site on MlaD

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK7187

   Plasmid

   #219761

   Purpose

   MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-p53DD

    Plasmid

    #196582

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK115

    Plasmid

    #219745

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D37E, V181I, S323M, M385K

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK7190

    Plasmid

    #219763

    Purpose

    MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    nnLuz, nnH3H, nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK7183

    Plasmid

    #219760

    Purpose

    MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PzPKS2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK7180

    Plasmid

    #219762

    Purpose

    MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    HmS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PE7-P2A-GFP

    Plasmid

    #222996

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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