We narrowed to 3,558 results for: mpo

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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-SF14P

Plasmid

#209448

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from SF14P promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-kasOP*

Plasmid

#209447

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from kasOP* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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CCM2_I432D_eGFP

Plasmid

#208879

Purpose

Express eGFP-CCM2 with the I432D mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutation I432D

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_W421AD422A_eGFP

Plasmid

#208880

Purpose

Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutations W421A and D422A

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_I428S_eGFP

Plasmid

#208878

Purpose

Express eGFP-CCM2 with the I428S mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutation I428S

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_g5-HT2h

Plasmid

#208715

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC28-HiBIT-WDR5

Plasmid

#200883

Purpose

Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-WDR5 (WDR5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E

Plasmid

#146211

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

Plasmid

#146212

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

Plasmid

#146213

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

Plasmid

#146215

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

Plasmid

#146216

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

Plasmid

#146204

Purpose

Insect Expression of DmMe31B-Q281AH284A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-Q281AH284A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

Plasmid

#146205

Purpose

Insect Expression of DmMe31B-T288AK292A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-T288AK292A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GA46

Plasmid

#196614

Purpose

Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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