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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Publication

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Publication

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCXLE-gw

Plasmid

#37626

Purpose

Non-integrating (episomal) expression vector with Gateway cassette

Depositor

Shinya Yamanaka

Publication

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Type

Empty backbone

Expression

Mammalian

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MSCV-Myc-IRES-RFP

Plasmid

#35395

Depositor

Martine Roussel

Publication

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
RFP

Use

Retroviral

Expression

Mammalian

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
Lact-C2-GFP-p416

Plasmid

#22853

Depositor

Sergio Grinstein

Publication

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2

Tags

GFP

Expression

Yeast

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Publication

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Mutation

C867A

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Publication

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pαH-S-GSAS-2P-N317P

Plasmid

#196378

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Publication

Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:

Insert

S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only
pCDNA-FLAG-METTL3

Plasmid

#160250

Purpose

Express FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-NSP2SA11

Plasmid

#89169

Purpose

Express Rotavirus SA11 NSP2 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP2

Expression

Mammalian

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Publication

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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hLRP1-pEZT-BM

Plasmid

#177985

Purpose

Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

Depositor

Lora Hooper

Publication

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein 1 (LRP1 Human)

Tags

No

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MinLibCas9

Pooled library

#164896

Purpose

Genome-wide knockout library designed with two sgRNAs per gene.

Depositor

Mathew Garnett

Publication

Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Publication

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Publication

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Publication

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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