We narrowed to 13,608 results for: crispr cas9

1. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

   Plasmid

   #184290

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Rxfp3
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

   Plasmid

   #184295

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Adrb1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT-Cas9-TMV

   Plasmid

   #238016

   Purpose

   for DNA-free gene knockout editing in rice and wheat or other plants

   Depositor

   Caixia Gao

   Article

   Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

   Insert

   SpCas9
   

   Expression

   Plant

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-Cas9-DEN2

   Plasmid

   #238017

   Purpose

   for DNA-free gene knockout editing in rice and wheat or other plants

   Depositor

   Caixia Gao

   Article

   Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

   Insert

   SpCas9
   

   Expression

   Plant

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-AAVS1_sgRNA

   Plasmid

   #183891

   Purpose

   pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1289-AAV-dCjCas9

   Plasmid

   #223138

   Purpose

   AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Cas9-2A-GFP

   Plasmid

   #170159

   Purpose

   Express Cas9 and GFP upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   Cas9
   

   Use

   Synthetic Biology; Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS415_pGal-nCas9(H840A)

    Plasmid

    #79616

    Purpose

    Expresses nCas9(H840A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Expression

    Yeast

    Mutation

    H840A for nickase Cas9

    Promoter

    pGal1

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-Cas9-tdTomato

    Plasmid

    #141013

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ciCas9(L22)_pcDNA5

    Plasmid

    #100551

    Purpose

    Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(L22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ciCas9(F22)_pcDNA5

    Plasmid

    #100552

    Purpose

    Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(F22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-ChBtW-eSpCas9

    Plasmid

    #133370

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Hyg-nCas9n

    Plasmid

    #217487

    Purpose

    Expression of nuclear nCas9n

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-CcuCas9

    Plasmid

    #192127

    Purpose

    Expresses CcuCas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    CcuCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-Hsp2Cas9

    Plasmid

    #192126

    Purpose

    Expresses Hsp2Cas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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