We narrowed to 8,660 results for: pcDNA3

1. pCDNA3-HA/Bmal L95E

   Plasmid

   #47344

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3 ATP5O(TISU)-FF

   Plasmid

   #85489

   Purpose

   Firefly luciferase under the control of ATP5O TISU

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   Full TISU element of ATP5O (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   2nd and 3rd codon of luciferase were modified fro…

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-DNM2-W525L

   Plasmid

   #155263

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tryptophan 525 to Leucine

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-SLAP-FLAG

   Plasmid

   #74500

   Purpose

   Overexpression of SLAP in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

   Insert

   SLAP (SLA Human)
   

   Expression

   Mammalian

   Mutation

   N18K, G74A, and T149A

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. Myc-gamma3 (in pCDNA3.1)

   Plasmid

   #118041

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-gamma1 (in pCDNA3.1)

   Plasmid

   #118038

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 HRPT2 136X

   Plasmid

   #11049

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   HRPT2 136X (CDC73 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncation mutant with a stop codon introduced at…

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-DKAR-Nuc

   Plasmid

   #45499

   Depositor

   Alexandra Newton

   Article

   Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

   Insert

   DKAR-Nuc
   

   Tags

   CFP, NLS, and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-gap16-ClopHensor

   Plasmid

   #26006

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   gap16-ClopHensor
   

   Tags

   Gap16 and Strep-II

   Expression

   Mammalian

   Mutation

   N-term (16AA) of Gap-43

   Available Since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CARD14-D176H

    Plasmid

    #69919

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    D176H

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-Nluc(W12stop)

    Plasmid

    #180502

    Purpose

    Mammalian expression of Nluc(W12stop)

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc(W12stop)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W12(UGG) to amber stop codon(UAG)

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Henry Lester, Jerry Stitzel

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. a4 ecto pcDNA3.1 hygro(-)

    Plasmid

    #113406

    Purpose

    expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

    Insert

    human alpha 4 ectodomain
    

    Tags

    Strep II tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-HA-ERK2 L198A

    Plasmid

    #8975

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

17. XE212 RGS9-pcDNA3.1

    Plasmid

    #16676

    Depositor

    Randall Moon

    Insert

    RGS9 (Rgs9 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-sACE2v2.2-IgG1

    Plasmid

    #149667

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG-Lin28BdelN70

    Plasmid

    #51374

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 70 amino acids deletion

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-Q842gp120-8his

    Plasmid

    #100932

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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