We narrowed to 4,410 results for: BRE

1. pIGc-Xph15

   Plasmid

   #133030

   Purpose

   Expresses Xph15 in bacteria

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph15
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   S63K

   Promoter

   T7

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3H HapII R175/6E-Cas9n-UGI

   Plasmid

   #119142

   Purpose

   Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   A3H Hap II RR175/6EE

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT 3xFLAG SMAD4

   Plasmid

   #83094

   Purpose

   Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   TRE tight promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 puro_shDDX58_230212

   Plasmid

   #167293

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA hsDDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SynaptoTAG2

   Plasmid

   #175275

   Purpose

   AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
   

   Use

   AAV

   Tags

   EGFP and tdTomato (P2A cleavage)

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-Cas9-P2A-Puro

   Plasmid

   #110837

   Purpose

   Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICE-HA-MRE11-H129N

   Plasmid

   #82034

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Nuclease-dead mutant: H129N MRE11

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-MRE11-H63D

    Plasmid

    #82036

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exonuclease-dead mutant: H63D MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-184_Cacnes_mCherry

    Plasmid

    #225610

    Purpose

    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEND-224_Cacnes_sfGFP

    Plasmid

    #225611

    Purpose

    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE Flag HA MPG

    Plasmid

    #237804

    Purpose

    Lentiviral vector expressing WT MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Tags

    Flag 3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    U6

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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