We narrowed to 7,819 results for: Lif

1. pJET-10-mCherry-R11b

   Plasmid

   #179891

   Purpose

   Donor with homologous arms for Rab11b to knock in mCherry

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-BTLA (PD-1 ITIM)-mGFP

   Plasmid

   #180818

   Purpose

   Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   BTLA (PD-1 ITIM) (BTLA Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH3453

   Plasmid

   #179577

   Purpose

   Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   BFP

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available since

   March 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJH2890

   Plasmid

   #179561

   Purpose

   Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-eGFP-OPTN S473A 513A

   Plasmid

   #175763

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S473A S513A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Halo-WIPI2B H85E K88E

   Plasmid

   #175032

   Purpose

   Expresses WIPI2B (H85E K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

   Depositor

   Andrea Stavoe

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2B (H85E K88E) (WIPI2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-uTEV1-SQSfl

   Plasmid

   #171010

   Purpose

   HiLITR protease with full-length SQS targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-SQS(FL)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3delta4 GPR3-V5

   Plasmid

   #172601

   Purpose

   Lentiviral plasmid expressing c-terminal V5-tagged GPR3

   Depositor

   Robert Screaton

   Article

   Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

   Insert

   G protein-Coupled Receptor 3 (GPR3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMiniT:myc-GluA2 TKIT donor

   Plasmid

   #169423

   Purpose

   Donor DNA for TKIT knockin of Myc to GluA2 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Myc (Myc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid

    #162654

    Purpose

    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJET1.2_Nkx3-2_Anti-Sense

    Plasmid

    #124438

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET1.2_Nkx3-2_Sense

    Plasmid

    #124437

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJET1.2_Rab28_Sense

    Plasmid

    #124441

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJET1.2_Rab28_Anti-Sense

    Plasmid

    #124442

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

    Plasmid

    #114373

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv4.2LL (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv4.2LL

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

    Plasmid

    #114375

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv2.1C

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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