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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-CreERT2

Plasmid

#14797

Purpose

Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pSMT3-M

Plasmid

#26589

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

n/a

Use

Shuttle vector for mycobacteria

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBAC-lacZ

Plasmid

#13422

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pNFkB:eGFP

Plasmid

#44922

Depositor

John Rawls

Article

Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

Insert

EGFP

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTH102

Plasmid

#84460

Purpose

pJB38-NWMN29-30 + SarA_P1-CFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-CFP from pTH2

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBsaBM3

Plasmid

#85102

Purpose

P450 BM3 variant (A74G/C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450 BM3

Use

Cloning vector

Mutation

A74G/C773S/C810S

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAB213

Plasmid

#101677

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRho-DsRed

Plasmid

#11156

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

rhodopsin promoter (RHO Bovine)

Tags

DsRed2

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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