We narrowed to 3,926 results for: SPL

1. pRS313 - prp8 V1870N

   Plasmid

   #90976

   Purpose

   yeast vector

   Depositor

   Christine Guthrie

   Article

   Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   V1870N

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS313 - prp8 I1875T

   Plasmid

   #90977

   Purpose

   yeast vector

   Depositor

   Christine Guthrie

   Article

   Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   I1875T

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. p414GPD-3xFLAG-RBM28 WT

   Plasmid

   #169265

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 WT (RBM28 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO - RBM28 E8 WT minigene

   Plasmid

   #169271

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 WT minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2-p21 promoter-Luc

   Plasmid

   #33021

   Depositor

   Martin Walsh

   Article

   Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

   Insert

   p21 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   p21

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

   Plasmid

   #205447

   Purpose

   human AAVS1-targeting plasmid for expression of mouse CR1

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV minimal

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPR-E

   Plasmid

   #78852

   Purpose

   Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

   Depositor

   Phillip Abbosh

   Article

   Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Cas9-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCh-LgBiT-2A-EGFP-Vin-PILATeS

    Plasmid

    #216761

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcI-ts,ind+-(mtSSB delta20)

    Plasmid

    #186280

    Purpose

    Expresses Human Mitochondrial SSB in E. coli

    Depositor

    Kenneth Johnson

    Article

    Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

    Insert

    Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    First 20 amino acids corresponding to probable mi…

    Promoter

    lambda

    Available since

    July 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAT15516_BEAR-GFP-2in1

    Plasmid

    #174082

    Purpose

    BEAR-GFP plasmid with a gRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Jizhong Zou, Mahendra Rao

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Jizhong Zou, Mahendra Rao

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO-Flag-H3.3

    Plasmid

    #47980

    Purpose

    tetracycline-inducible expression of human Flag tagged H3F3A

    Depositor

    Bing Zhu

    Article

    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

    Insert

    H3F3A (H3-3A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    288 bp T to C synonymous mutation

    Promoter

    CMV promoter with tetracycline operator

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Use

    Tags

    GFP11x7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLHCX-FLAG-mPKM1

    Plasmid

    #44240

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M1 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only