We narrowed to 9,387 results for: pcas

1. pD649-HAsp-EPCAM-COMP5AP-AviTag-9xHis

   Plasmid

   #157257

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EPCAM (EPCAM Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-EPCAM-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156693

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EPCAM (EPCAM Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

   Plasmid

   #128166

   Purpose

   Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   mTagBFP2-T2A-sfGFP-iCre-ERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108845

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GAI

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108843

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GID1

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

   Plasmid

   #108814

   Purpose

   Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   TP901 integrase fragment aa327 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108847

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    pMag

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108837

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108841

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    PYL

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-WIPI2d10-364delta263-295-TEV-STREP

    Plasmid

    #171830

    Purpose

    Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    10-364delta263-295

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69226

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108839

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

    Plasmid

    #113162

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA

    Plasmid

    #108824

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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