We narrowed to 7,267 results for: trac

1. pTBL516 CT sgRNA

   Plasmid

   #126433

   Purpose

   To target a protospacer with CT at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with CT at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTBL515 AA sgRNA

   Plasmid

   #126426

   Purpose

   To target a protospacer with AA at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with AA at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTBL509 AT sgRNA

   Plasmid

   #126429

   Purpose

   To target a protospacer with AT at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with AT at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTBL511 CC sgRNA

   Plasmid

   #126431

   Purpose

   To target a protospacer with CC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with CC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTBL508 TA sgRNA

   Plasmid

   #126438

   Purpose

   To target a protospacer with TA at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TA at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTBL250 TC sgRNA

   Plasmid

   #126439

   Purpose

   To target a protospacer with TC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTBL514 TG sgRNA

   Plasmid

   #126440

   Purpose

   To target a protospacer with TG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTBL513 TT sgRNA

   Plasmid

   #126441

   Purpose

   To target a protospacer with TT at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TT at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTBL322 GC

   Plasmid

   #126950

   Purpose

   To target a protospacer with GC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTBL581 GA

    Plasmid

    #126951

    Purpose

    To target a protospacer with GA at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with GA at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTBL601 AG

    Plasmid

    #126952

    Purpose

    To target a protospacer with AG at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with AG at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBL602 CG

    Plasmid

    #126953

    Purpose

    To target a protospacer with CG at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with CG at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-kanMX6-P3nmt1-mECitrine

    Plasmid

    #105151

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    kanMX6-P3nmt1-mECitrine
    

    Use

    Tags

    3nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    3nmt1

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH009

    Plasmid

    #80399

    Purpose

    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain and N-terminal 3xFLAG tag, …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30-SBL1(160S,HisTagDel)

    Plasmid

    #66880

    Purpose

    Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (S160S)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET30-SBL1(160E,HisTagDel)

    Plasmid

    #66879

    Purpose

    Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (S160E)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge