Addgene

We narrowed to 19,664 results for: ACE

Showing: 221 - 240 of 19664 results

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pPD062 ZF43x2-Spaced_EYFP

Plasmid

#138875

Purpose

EYFP reporter vector for the ZF43x2-Spaced promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x2-Spaced

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPD059 ZF43x1-Spaced_EYFP

Plasmid

#138874

Purpose

EYFP reporter vector for the ZF43x1-Spaced promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x1-Spaced

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4-4T-1 Acetobacter AGSC

Plasmid

#161813

Purpose

Expresses AGSC domain from Acetobacter type I SMODS/CD-NTase enzyme

Depositor

Douglas Feldman

Insert

AGSC domain Acetobacter SMODS/CD-NTase enzyme

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2(732)

Plasmid

#154099

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v4-Fc(IgA1)

Plasmid

#145159

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-T92Q

Plasmid

#145156

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Use

Tags

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-pegA + aceS

Plasmid

#114457

Purpose

Expression of PegA and AceS in streptomycetes

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

pegA + aceS

Use

Tags

Expression

Bacterial

Mutation

Promoter

ermE

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Use

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAce-Kv2.1PR

Plasmid

#195529

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Use

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha_short (EFS)

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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hBACE1(D289N).ires.mCherry-pcDNA3

Plasmid

#204363

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

BACE1 (BACE1 Human)

Use

Tags

Expression

Mammalian

Mutation

D289N

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Use

Tags

His8 and xMBP

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only