-
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322_ZA-ampR-PSEAB-2A
Plasmid#135230PurposeAAC6 experimental evolutionDepositorInsertaac6 Pseudomonas sp. ABAC61
UseTagsExpressionBacterialMutationPromoterpZAAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJPT008-ZF6 M-synTF-1x FLAG
Plasmid#202498PurposeExpress "M" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Myristoylation siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT007-ZF6 synTF-1x FLAG
Plasmid#202497PurposeExpress "soluble" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT006-1x FLAG-HaloTag-PPF
Plasmid#202496PurposeExpress "PPF" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Palmitoylation-Palmitoylation-Farnesy…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT010-ZF6 1x FLAG-synTF-G
Plasmid#202500PurposeExpress "G" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT004-1x FLAG-HaloTag-G
Plasmid#202495PurposeExpress "G" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-NgPglO
Plasmid#198204PurposepSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tagDepositorInsertNgPglO
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*-(ΔLF)
Plasmid#80045PurposeExpression of Vpr binding mutant EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationVpr binding site LF45 in p6 mutatedPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1+L2)
Plasmid#80051PurposeExpression of EPN-01 mutated in both L domainsDepositorInsertEPN-01(deltaPTAP/deltaYP)
UseTagsMycExpressionMammalianMutationPTAP10AAAA, YP37AAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔI)
Plasmid#80041PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationchanged Leu33 in I3-01 to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only