We narrowed to 58,531 results for: SAP

1. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

   Plasmid

   #233031

   Purpose

   To express a DjCas13d-compatible gRNA

   Depositor

   Jonathan Ploski

   Insert

   DjCas13d-compatible gRNA expression cassette
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

   Plasmid

   #233038

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5'-BirA-FLAG Sap30bp-exon minimal CMV pCDNA5

   Plasmid

   #212092

   Purpose

   LR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Sap30bp-exon (Sap30bp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5'-BirA-FLAG Sap30bp+exon minimal CMV pCDNA5

   Plasmid

   #212093

   Purpose

   LR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Sap30bp+exon(48nt) (Sap30bp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

   Plasmid

   #176589

   Purpose

   Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Chesapeake Bay) locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated CRISPR array to two direct repeats, rep…

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233046

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

   Plasmid

   #233045

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

   Depositor

   Jonathan Ploski

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

   Plasmid

   #196895

   Purpose

   Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

   Plasmid

   #192161

   Purpose

   AAV-CRISPRa base vector

   Depositor

   Sidi Chen

   Article

   Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid

    #113700

    Purpose

    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid

    #113698

    Purpose

    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

    Plasmid

    #190898

    Purpose

    Entry vector for multiple sgRNA cloning

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO EYFP (AAV5)

    Viral Prep

    #27056-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-DIO EYFP (AAV9)

    Viral Prep

    #27056-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Ef1a-DIO EYFP (AAV1)

    Viral Prep

    #27056-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-eNpHR 3.0-EYFP (AAV5)

    Viral Prep

    #26972-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-eNpHR 3.0-EYFP (#26972). In addition to the viral particles, you will also receive purified pAAV-hSyn-eNpHR 3.0-EYFP plasmid DNA. hSyn-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-Ef1a-DIO EYFP (AAV2)

    Viral Prep

    #27056-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

    Viral Prep

    #26971-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    EYFP

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV1)

    Viral Prep

    #26971-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    EYFP

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-hSyn-hChR2(H134R)-EYFP (AAV9)

    Viral Prep

    #26973-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only