We narrowed to 962 results for: cas12a

1. SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

   Plasmid

   #244213

   Purpose

   Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-KRAB
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastR

   Plasmid

   #244211

   Purpose

   Mammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dHyperLbCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

   Plasmid

   #244216

   Purpose

   Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-P300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

   Plasmid

   #244215

   Purpose

   Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-4xSID
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

   Plasmid

   #244214

   Purpose

   Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker

   Depositor

   Tim Reddy

   Article

   Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

   Insert

   dEnAsCas12a-4xSID
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

   Plasmid

   #182126

   Purpose

   pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

   Plasmid

   #182121

   Purpose

   pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

   Plasmid

   #182124

   Purpose

   pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
   TRAC-3' homology arm (TRAC Human)
   

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

    Plasmid

    #114067

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for AsCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAST-FRT-GFP-FRT-uMCas12a+nls1x

    Plasmid

    #230908

    Purpose

    Double inducible expression of Cas12a+ with Gal4 and Flp.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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