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pHR- flag-Lag43-iCAM-1

Plasmid

#205206

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG43

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag43 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E143amb)-His

Plasmid

#168046

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 143 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMK-PatpI (PEP Hypothetical)

Plasmid

#123473

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PatpI (PEP Hypothetical)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-SIRPT1-Loxodonta Africana

Plasmid

#75319

Purpose

GST fusion Loxodonta Africana Sacsin 89-1368

Depositor

Kalle Gehring

Insert

Sacsin Loxodonta Africana 89-1368

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Radical Fringe (CT#475)

Plasmid

#13981

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) Radical Fringe (CT#476)

Plasmid

#13982

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

Plasmid

#200630

Purpose

Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

iCasp9

Use

Lentiviral

Promoter

pTRE3G

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-iCAM-1-Tether

Plasmid

#205187

Purpose

This vector encodes of a synCAM (iCAM1 Tether) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 tether and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-Flag-APH4-Fibcon-icam

Plasmid

#233077

Purpose

Expresses synCAM-APH4-Fibcon-icam

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

APH4-FIBCON-ICAM

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-iCAM-1-Tether

Plasmid

#205196

Purpose

This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 Tether and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-fibcon-iCAM1

Plasmid

#205212

Purpose

This vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Xenopus (Silurana) tropicalis

Plasmid

#75355

Purpose

Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

Depositor

Kalle Gehring

Insert

Sacsin Xenopus (Silurana) tropicalis 87-1376

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-Flag-IF1-Fibcon-icam

Plasmid

#233076

Purpose

expresses synCAM -IF1-fibcon - iCAM1

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

If1-Icam

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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