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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

Plasmid

#225698

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-Zf2

Plasmid

#160944

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 2 (C306 to C330)

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-Zf1

Plasmid

#160943

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 1 (C270 to C290)

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-AICD-IRES-hrGFP

Plasmid

#107548

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD (APP Human)

Use

AAV

Mutation

None

Promoter

human synapsin

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD

Plasmid

#225872

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3(K36M) amino acids 1-44 (H3-3A, H3-3B Synthetic, Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

amino acids 1-44 from H3.3 K36M

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBN49

Plasmid

#35131

Depositor

John McCusker

Article

Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

Insert

hygMX4/T-urf13

Use

Yeast replicating

Available Since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only
pSA41

Plasmid

#35130

Depositor

John McCusker

Article

Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

Insert

natMX4

Use

Yeast replicating

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pBN98

Plasmid

#35133

Depositor

John McCusker

Article

Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

Insert

kanMX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pBN33

Plasmid

#35132

Depositor

John McCusker

Article

Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

Insert

fcy1-natMX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
LGhP-pHG165

Plasmid

#90065

Purpose

Constitutive expression of a chimeric LacI:GalR:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al PLoS One. 2013 Dec 30;8(12):e83502. doi:

Insert

LGhP

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_497

Plasmid

#111824

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 477-497

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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LLhG-pHG165a E62K

Plasmid

#90051

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

Insert

LLhG/E62K

Expression

Bacterial

Mutation

E62K

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_191

Plasmid

#111821

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 171-191

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_369

Plasmid

#111823

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 349-369

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge