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We narrowed to 282 results for: mCitrine

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Showing: 221 - 240 of 282 results

  2. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Publication
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  4. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Publication
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

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  8. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Publication
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  9. mCit-Snap25b D179K A199R

    Plasmid
    #53259
    Purpose
    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    D179K and A199R
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pGEM-PH-citrine

    Plasmid
    #131406
    Purpose
    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
    Depositor
    Lars Hufnagel
    Publication
    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369
    Insert
    PH
    Use
    In vitro transcription
    Tags
    citrine yellow fluorescent protein
    Promoter
    T7
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131406-plasmid-map-sequence-id-261478

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  13. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Expression
    Mammalian
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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  20. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Expression
    Mammalian
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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Showing: 221 - 240 of 282 results