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pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation F (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
SNX15-PX (1-127)

Plasmid

#119096

Purpose

Bacterial expression of human phox homology (PX) domain, SNX15-PX (1-127)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX15-PX (1-127) (SNX15 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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p4nmTriangle

Plasmid

#250946

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 4nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

4nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p20nmTriangle

Plasmid

#250947

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 20nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

20nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p60nmTriangle

Plasmid

#250948

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 60nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

60nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ134B-TLS

Plasmid

#245879

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-PtALSgRNA

Plasmid

#245880

Purpose

Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

PtALSgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ133B-TLS

Plasmid

#245882

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_KO_Puro_donor

Plasmid

#246393

Purpose

MTF2 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_Puro_donor

Plasmid

#246399

Purpose

PHF19 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_PHF1_sgRNA

Plasmid

#246403

Purpose

Cas9/sgRNA expression plasmid targeting PHF1

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_PHIP

Plasmid

#240328

Purpose

Entry vector for Gateway with PHIP

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

PHIP (PHIP Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7

Plasmid

#240319

Purpose

Entry vector for Gateway with USP7

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroLITE

Plasmid

#215614

Purpose

For integration of CEBPa AroLITE mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroLITE (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Prepro-3x-Adan

Plasmid

#181733

Purpose

Galactose inducible expression of Prepro-3x-Adan

Depositor

Alejandro Chavez

Insert

Prepro-3x-Adan

Expression

Yeast

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_D146N_E45Q

Plasmid

#210798

Purpose

pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D146N_E45Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_F298A_T299A_H300A

Plasmid

#210799

Purpose

pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F298A_T299A_H300A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_MsMutY_F302A_T303A_H304A

Plasmid

#210795

Purpose

pkk223 with Marinosulfonomonas MutY containing F302A, T303A, and H304A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F302A_T303A_H304A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_MsMutY_D150N_E49Q

Plasmid

#210794

Purpose

pkk223 with Marinosulfonomonas MutY containing D150N and E49Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D150N_E49Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_F305A_S306A_H307A

Plasmid

#210793

Purpose

pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F305A_S306A_H307A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_D147N_E46Q

Plasmid

#210792

Purpose

pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D147N_E46Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8A1-HA

Plasmid

#209225

Purpose

Mammalian expression of ATP8A1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST ɑSynWT-EGFP

Plasmid

#201249

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

Insert

ɑ-Synuclein (SNCA Human)

Expression

Insect

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Tags

2XGFP

Expression

Worm

Promoter

nlf-1

Available Since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET-CV-VAMP5

Plasmid

#168026

Purpose

Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP5(M1-Q60)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_hTLN2-FL-LBD

Plasmid

#164841

Purpose

Expression of GFP tagged FL human talin2 mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-GFP

Expression

Mammalian

Mutation

Lipid binding deficient mutations

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-N342W

Plasmid

#160518

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

N342W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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