We narrowed to 18,722 results for: IRE

1. pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85179

   Purpose

   For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) tagRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL3.7 K122 FH-TAZ-ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

   Plasmid

   #68713

   Depositor

   Yutaka Hata

   Insert

   H2B mCherry and TAZ/WWTR1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for TAZ/WWTR1

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

   Plasmid

   #122986

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-SspB(micro)-BoNT/B(147-441)
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   SspB contains R73Q "micro" mutation, Bo…

   Promoter

   human synapsin

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

   Plasmid

   #238166

   Purpose

   Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

   Depositor

   Thomas Norman

   Article

   Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

   Insert

   VPH-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

   Plasmid

   #231237

   Purpose

   MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NES fused to the DNA-binding-domain of MYC (resid…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

   Plasmid

   #231236

   Purpose

   MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLS fused to the DNA-binding-domain of MYC (resid…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 VOC-501Y.V2 (B.1.351) - McGuire Mutations

   Plasmid

   #212388

   Purpose

   Encodes SARS-CoV-2 spike D80A,D215G,K417N,E484K,N501Y,D614G for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D80A,D215G,K417N,E484K,N501Y,D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D80A,D215G,K417N,E484K,N501Y,D614G

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405A

   Plasmid

   #188138

   Purpose

   Expresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

   Plasmid

   #183396

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain

    Plasmid

    #183395

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDAX-483 GFP[1] L9 Stuffer/Intein3 L10 GFP[2] IRES mCherry GFP(1-9)

    Plasmid

    #130166

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDAX-484 GFP[1] L9 Stuffer/Intein4 L10 GFP[2] IRES mCherry GFP(1-9)

    Plasmid

    #130167

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDAX-482 GFP[1] L9 Stuffer/Intein2 L10 GFP[2] IRES mCherry GFP(1-9)

    Plasmid

    #130165

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDAX-481 GFP[1] L9 Stuffer/Intein1 L10 GFP[2] IRES mCherry GFP(1-9)

    Plasmid

    #130164

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid

    #214282

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid

    #209081

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T, NAC deletion (aa60-95)

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214278

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

    Plasmid

    #183397

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid

    #122955

    Purpose

    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of EGF repeats from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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