We narrowed to 2,986 results for: KIS

1. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

   Plasmid

   #166693

   Purpose

   Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9-VPR
   3x Cnga1 promoter-targeting sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and U6

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL-Neo-Myr-Flag-DEST

   Plasmid

   #15300

   Purpose

   a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

   Depositor

   Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Archon1-KGC-EGFP-ER2-WPRE

   Plasmid

   #108423

   Purpose

   Archon1 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ALK6 Q203D

   Plasmid

   #80883

   Purpose

   mammalian expression of ALK6 Q203D

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q203D (constitutively active)

   Promoter

   CMV

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-ALK6 K231R

   Plasmid

   #80884

   Purpose

   mammalian expression of ALK6 K231R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K231R (kinase-deficient)

   Promoter

   CMV

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-ARAP3

   Plasmid

   #39484

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3 (ARAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSF143-wArchon1-KGC-EGFP-ER2

   Plasmid

   #108429

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   wArchon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   flp-18

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2/shCol1a1/GFP4_Seq1.3

   Plasmid

   #206136

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1 (Col1a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Puro-Myr-Flag-IKBKE

   Plasmid

   #15293

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Myr-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CaMKII-Archon1-EGFP

    Plasmid

    #108417

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-EF1a-Archon1-KGC-EGFP-ER2

    Plasmid

    #108425

    Purpose

    Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-FLAG-TAPBPR-TM-VC

    Plasmid

    #135499

    Purpose

    Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal/Extracellular FLAG epitope tag, Signal pe…

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Archon2-KGC-GFP-ER2-WPRE

    Plasmid

    #108424

    Purpose

    Archon2 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-Puro-Flag-IKBKE

    Plasmid

    #15292

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Neo-Flag-IKBKE

    Plasmid

    #15265

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pBabe-Puro-cRAF-W22

    Plasmid

    #15270

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pmiRFP-N1

    Plasmid

    #108409

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol2-UAS-zmiRFP

    Plasmid

    #108415

    Purpose

    Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zmiRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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