We narrowed to 23,463 results for: his

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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ppr_Cas13a_WT

Plasmid

#91872

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

PprCas13a (PprC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1-GFP

Plasmid

#111657

Purpose

C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1-GFP (Bicdl1 Mouse)

Tags

8xHis-tag, GFP, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEB31 (hANGPTL8-V5-His lenti)

Plasmid

#109116

Purpose

V5 and His-tagged human ANGPTL8 in a lentiviral vector

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (ANGPTL8 Human)

Use

Lentiviral

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mTurquoise2-Tub1+3'UTR::HPH

Plasmid

#50629

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mTurquoise2-Tub1

Use

Yeast expression

Tags

mTurquoise2

Promoter

HIS3p

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his24::tagBFP2

Plasmid

#124344

Purpose

Nuclear Localized Pan-Neuronal tagBFP2

Depositor

Andrew Leifer

Insert

rab3, tagBFP2 (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPH3

Plasmid

#19536

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4-like (HSPA4L Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-Myc-HIS3MX6

Plasmid

#20768

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

Plasmid

#79259

Purpose

Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmeIF4G (eIF4G1 Fly)

Use

Schneider 2 cell expression

Tags

V5-His6 and lambdaN-HA

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT 5N5A

Plasmid

#154280

Purpose

Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

N322A, N356A, N390A, N424A, N458A mutations; bact…

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA2

Plasmid

#19539

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB9

Plasmid

#19559

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9 C287A-His

Plasmid

#11830

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytic mutant (CASP9 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C287A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAS1-odr-4P::his-24::mNeptune

Plasmid

#124342

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

odr-4, mNeptune (odr-4 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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