We narrowed to 8,272 results for: reporter

1. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCB619

   Plasmid

   #171011

   Purpose

   CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   U6:sgRNA_Puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

   Plasmid

   #184395

   Purpose

   Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215328

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   FOXJ1 Promoter
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FOXJ1

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-uTEV1-CB5

   Plasmid

   #171007

   Purpose

   HiLITR protease with CB5 C-terminal tmd targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-CB5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetO-FUW-Rfx4

   Plasmid

   #61544

   Purpose

   Expresses Rfx4 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Rfx4 (Rfx4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pSCMV-H2B-FT-Medium

   Plasmid

   #157670

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Medium-FT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cox7c-pHLuorin-sems

    Plasmid

    #107766

    Purpose

    labeling of mitochondrial cytochrome c oxidase, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit Cox7c fused to pHluorin (COX7C Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45564

    Purpose

    Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    d14 eGFP donor
    I-Sce I Nuclease
    BFP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted n' 15 amino acids

    Promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCMV-H2B-FT-Fast

    Plasmid

    #157669

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Fast-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCMV-H2B-FT-Slow

    Plasmid

    #157671

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Slow-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Use

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

    Plasmid

    #184396

    Purpose

    Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge