We narrowed to 6,862 results for: GFP expression plasmids

1. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

   Plasmid

   #185910

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

   Plasmid

   #185915

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)

   Plasmid

   #39302

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D88A, K89A, D94A and K95A

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

5. human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

   Plasmid

   #39303

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q109A, W110A, K112A and K113A

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)

   Plasmid

   #39304

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K122A, D123A, Y124A and D125A

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. AAVS1-Pur-CAG-EGFP

   Plasmid

   #80945

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAVS1-Puro Xlone-eGFP

   Plasmid

   #136936

   Purpose

   donor plasmid for inducible transgene expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   all-in-one tet-on system
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV2-CMV-GFP-BGHpA

    Plasmid

    #190239

    Purpose

    Expresses EGFP in mammalian cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPRIME-TET-GFP-recipient

    Plasmid

    #11655

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 7b sgRNA for EJ7-GFP reporter

    Plasmid

    #113624

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7b sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pLV-EF1a-GFP ArgSen

    Plasmid

    #184704

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PZac2.1 hSyn1-Lck-GFP

    Plasmid

    #177333

    Purpose

    Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Lck-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    hSyn1

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-CamKIIa-ChR2-EGFP

    Plasmid

    #114216

    Purpose

    Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Channelrhodopsin 2
    

    Use

    AAV and Mouse Targeting

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa 1.3 kb

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-BRX-eGFP-NLS

    Plasmid

    #217534

    Purpose

    AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    BMP reporter element and miniXon casette (Smad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BMP Reporter Element

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTP924 pHAGE2 CMV GFP

    Plasmid

    #199392

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEXfrt-EGFP-KASH

    Plasmid

    #154374

    Purpose

    Vector for Flp-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV2-SYN-GFP-BGHpA

    Plasmid

    #190229

    Purpose

    Expresses EGFP in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge