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pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

Plasmid

#157277

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF6B (TNFRSF6B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666A

Plasmid

#105310

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP(TE)

Plasmid

#61522

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TE mutation (see comments), Tom20, and m…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc-mβTrCP F-box (1-247)

Plasmid

#62976

Purpose

Expresses myc-tagged mouse βTrCP residues 1-247

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

3x myc

Expression

Mammalian

Mutation

codes for residues 1-247

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-LAIR2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156520

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LAIR2 (LAIR2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgMARK4

Plasmid

#138691

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-HA-GFP11-KDEL

Plasmid

#177724

Purpose

ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Tags

GFP11, HA, KDEL, and Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148884)

Plasmid

#77194

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
Mc4r->M71-IRES-tauGFP ACNF TV

Plasmid

#105072

Purpose

Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassette

Depositor

Peter Mombaerts

Article

Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

Insert

Mc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)

Use

Mouse Targeting

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001149479)

Plasmid

#77454

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001145017)

Plasmid

#77455

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKG gRNA (BRDN0001147060)

Plasmid

#77456

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKG (DGKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001144986)

Plasmid

#75515

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001145395)

Plasmid

#75516

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001145877)

Plasmid

#75517

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001148333)

Plasmid

#75518

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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