We narrowed to 7,175 results for: ef1a

1. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132407

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLwaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R474A; H479A; R1046A; R1051A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. retro-gRNA-eGFP

   Plasmid

   #116926

   Purpose

   Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

   Depositor

   Christophe Benoist, Diane Mathis

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

   Plasmid

   #132401

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132408

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLbaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R600A; H605A; R1243A; H1248A

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132399

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. retro-gRNA-mRFP1

   Plasmid

   #112914

   Purpose

   Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

   Depositor

   Christophe Benoist, Diane Mathis

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   mRFP1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-DIO-IGK-dAPEX2-KDEL

   Plasmid

   #117183

   Purpose

   Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBA409

    Plasmid

    #85971

    Purpose

    EF1a-sfGFP-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sfGFP
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSH-EFIRES-P-AtAFB2

    Plasmid

    #129715

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    no

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-MSK1

    Plasmid

    #165601

    Purpose

    Expresses Sp dCas9 fused to human MSK1

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    ribosomal protein S6 kinase A5 (RPS6KA5 Synthetic, Human, S. Pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS133

    Plasmid

    #211504

    Purpose

    AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    mTagBF2
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-Bos MAL Flag

    Plasmid

    #41554

    Depositor

    Tom Maniatis, Kate Fitzgerald

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    MAL (TIRAP Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pMJK2243

    Plasmid

    #227688

    Purpose

    Empty lentivector to overexpress gene of interest

    Depositor

    Curt Civin

    Article

    Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS134

    Plasmid

    #211505

    Purpose

    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    Venus
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS132

    Plasmid

    #211503

    Purpose

    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    mCherry
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge