We narrowed to 9,247 results for: CAG

1. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

   Plasmid

   #140000

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

   Plasmid

   #196885

   Purpose

   Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

   Plasmid

   #108770

   Purpose

   Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa397 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #84445

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

   Plasmid

   #108735

   Purpose

   Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa256
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA

   Plasmid

   #108789

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108790

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

   Plasmid

   #69048

   Purpose

   lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   splice variant 1, Q146H

   Promoter

   CAG

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA

   Plasmid

   #108743

   Purpose

   Expresses N-terminus to aa256 of Cre recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa256
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA

    Plasmid

    #108744

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid

    #180644

    Purpose

    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    ULK1 (ULK1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    Residues 833-1050

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

    Plasmid

    #85799

    Purpose

    donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    homology arms for knock-in into first intron

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA

    Plasmid

    #108771

    Purpose

    Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa172
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA

    Plasmid

    #108772

    Purpose

    Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa173 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

    Plasmid

    #195101

    Purpose

    Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    AID, HA, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

    Plasmid

    #180486

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    VARNAM A122D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA

    Plasmid

    #108816

    Purpose

    Expresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Bxb1 integrase fragment aa469 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA

    Plasmid

    #108797

    Purpose

    Expresses N-terminus to aa233 of PhiC31 integrase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa233
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73475

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only