We narrowed to 281,368 results for: Des

1. pDest Lenti CA-GFP

   Plasmid

   #197459

   Purpose

   Bicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.

   Depositor

   Omar Khan

   Article

   Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S65T

   Promoter

   CMV, IRES

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST51-LRRK2-R1441C

   Plasmid

   #25081

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.2-V5-DEST-hSFRP1-VF

   Plasmid

   #189542

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-EGFP-TARG1

   Plasmid

   #172586

   Purpose

   For transient expression of N-terminal GFP-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST27-alpha-catenin

   Plasmid

   #20139

   Depositor

   Valeri Vasioukhin

   Article

   Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

   Insert

   pDEST27-alpha catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pLenti4/V5-DEST-P110alpha

   Plasmid

   #40230

   Depositor

   Lynda Chin

   Insert

   P110 alpha (PIK3CA Bovine)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hyper7 in pT3TS-Dest

   Plasmid

   #194296

   Purpose

   In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST_FLAG-HUWE1_UBM-mut

   Plasmid

   #187150

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST_FLAG-HUWE1_UIM-mut

    Plasmid

    #187148

    Purpose

    mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    A1376G/S1382A

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST_FLAG-HUWE1_UBA-mut

    Plasmid

    #187146

    Purpose

    mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    M1328A/F1330A

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. IQ013: pMVP/Ad-DEST

    Plasmid

    #121843

    Purpose

    pMVP destination vector, empty adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 726-Desmoglein2-myc

    Plasmid

    #32232

    Depositor

    Kathleen Green

    Article

    Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

    Insert

    Desmoglein2 (DSG2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    human beta-actin

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. 371 pTol2-fli1ep:MTDest

    Plasmid

    #73490

    Purpose

    Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    fli1ep:6xMyc-attR1-attR2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBdest-GST-PI3Kc1

    Plasmid

    #187992

    Purpose

    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

    Depositor

    Sascha Martens

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. KX002: pMVP/PB-DEST

    Plasmid

    #121869

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-DEST-Myc

    Plasmid

    #119752

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST47-ARL1-GFP

    Plasmid

    #67395

    Purpose

    Mammalian expression of hArl1 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL1 (ARL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    bp 231 C to T; silent mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDEST40-2XFL-Src-KD

    Plasmid

    #140313

    Purpose

    Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST51-LRRK2-R1441G

    Plasmid

    #25082

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arginine 1441 mutated to Glycine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only