We narrowed to 3,926 results for: SPL

1. DDX5 D248N V5

   Plasmid

   #199577

   Purpose

   Expresses helicase dead DDX5 cloned into pLenti puro

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   D248N

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-evoA1-seBEN_empty

   Plasmid

   #174701

   Purpose

   Small-molecule controlled base editing

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

   Insert

   Lenti-evoA1-seBEN
   

   Use

   Lentiviral

   Tags

   myc tag

   Expression

   Mutation

   Promoter

   EFS/U6

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-SF3B1-NTerm

   Plasmid

   #97425

   Purpose

   Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

   Depositor

   Alex Minella

   Article

   Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

   Insert

   SF3B1-N-term (AA1-500)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Silent mutation at nt876, aa289 (AGG to AGA)

   Promoter

   tac

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry

   Plasmid

   #133322

   Purpose

   Bacterial expression of TDP43 with FYW-S mutant CTD

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All FYW in the TDP43 IDRs mutated to S

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_SNORD27h

   Plasmid

   #73066

   Purpose

   expression clone for human SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (SNORD27 Human)
   

   Use

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   expression cassette for SNORD27

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. p199 Dync1i2.C (Ex1a)

   Plasmid

   #26449

   Depositor

   Elizabeth Fisher

   Article

   Kuta et al PLoS One. 2010 . 5(7):e11682.

   Insert

   cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)
   

   Use

   Subcloning and sequencing

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   March 24, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pET26b_Nb.AD102

   Plasmid

   #131403

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD102
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER21-puro_TAOK1

   Plasmid

   #209777

   Purpose

   doxycycline-inducible expression of TAOK1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK1 (TAOK1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_sgGFP4

    Plasmid

    #119876

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_nV5-TAOK2

    Plasmid

    #173000

    Purpose

    constitutive expression of V5-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSicoR-Ef1a-mCh-GFPi

    Plasmid

    #31849

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

    Plasmid

    #107858

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 2) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CFP-PBD(H83,86L)

    Plasmid

    #11402

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    p21-activated kinase 1 (PAK1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Obtained with Histidine residues 83 and 86 change…

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. tol2-GFAP::CD14

    Plasmid

    #59593

    Purpose

    Magnetic cell sorting of ES cell-derived astrocytes

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    human CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    gfa2

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. GFP-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #79676

    Purpose

    mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L182A/D183A/I184A/G185A catalytic mutant

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

19. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXPR_sgGFP3

    Plasmid

    #119875

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge