We narrowed to 3,070 results for: NAK

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pFAST-BAC BAF57/SMARCE1-His

Plasmid

#177861

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCE1 (SMARCE1 Human)

Tags

His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Ab

Plasmid

#128332

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform b

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry

Plasmid

#202818

Purpose

Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Expression

Mammalian

Mutation

addition of a dual aptamer RNA tag 5' to the…

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3_ mTFP1- Vamp2

Plasmid

#201214

Purpose

AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Insert

mTFP1 (from addgene #54613)

Use

AAV

Tags

mouse Vamp2

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pX330 Ctnnb1.2

Plasmid

#59912

Purpose

pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pbG-APRT-J-u32uni-PdTKmCh

Plasmid

#107279

Purpose

APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

APRT (APRT Human)
puro-deltaTK; CAG::mCherry

Mutation

c.402A>T, c.407T>C

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-Blast

Plasmid

#87155

Purpose

lentiviral expression of SpCas9-VQR (NGA PAM restricted)

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

SpCas9-VQR(D1135V,R1335Q,T1337R)

Use

Lentiviral

Mutation

D1135V,R1335Q,T1337R

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S586A-VLCAD-FLAG

Plasmid

#55824

Purpose

Expresses S586A VLCAD mutant in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Tags

FLAG tag

Expression

Mammalian

Mutation

changed Serine 586 toAlanine

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

T301N/N323S

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4

Plasmid

#136613

Purpose

Dox-inducible lentiviral vector expressing mouse Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone3

Plasmid

#162130

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Left TALE

Plasmid

#196496

Purpose

Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Right TALE

Plasmid

#196497

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104R-PdTK-mCh

Plasmid

#107276

Purpose

HPRT1 c.312C>A Munich donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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