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Plasmid#109832PurposeProtein expression and purification of DEPDC5_DEPDepositorInsertDEPDC5_DEP (DEPDC5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GIGYF1_GYF
Plasmid#109833PurposeProtein expression and purification of GIGYF1_GYFDepositorInsertGIGYF1_GYF (GIGYF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DEPDC1B_DEP
Plasmid#109814PurposeProtein expression and purification of DEPDC1B_DEPDepositorInsertDEPDC1B_DEP (DEPDC1B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARPC1A_WD40
Plasmid#109784PurposeProtein expression and purification of ARPC1A_WD40DepositorInsertARPC1A_WD40 (ARPC1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK4_IRS
Plasmid#109788PurposeProtein expression and purification of DOK4_IRSDepositorInsertDOK4_IRS (DOK4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MYL6_EF-hand
Plasmid#109794PurposeProtein expression and purification of MYL6_EF-handDepositorInsertMYL6_EF-hand (MYL6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK5_IRS
Plasmid#109797PurposeProtein expression and purification of DOK5_IRSDepositorInsertDOK5_IRS (DOK5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK1_IRS
Plasmid#109779PurposeProtein expression and purification of DOK1_IRSDepositorInsertDOK1_IRS (DOK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 S135A
Plasmid#155318PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104A/M247C)
Plasmid#155133PurposeExpresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/M247C, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
D19_Ld_130940
Plasmid#135835PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertsubtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorInsertshNts(2) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorInsertshNts(1) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DEPTOR_DEP
Plasmid#109882PurposeProtein expression and purification of DEPTOR_DEPDepositorInsertDEPTOR_DEP (DEPTOR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
UseTags6His-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only