Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,488 results for: PORA

Clear keywords
Showing: 2461 - 2480 of 2488 results

  3. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid
    #238169
    Purpose
    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    CD45 sgRNA CRISPRa (PTPRC Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238169-plasmid-map-sequence-id-474451

    Enlarge

  4. VEGF120TAG

    Plasmid
    #200946
    Purpose
    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200946-plasmid-map-sequence-id-396320

    Enlarge

  5. VEGF164TAG

    Plasmid
    #200947
    Purpose
    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200947-plasmid-map-sequence-id-396321

    Enlarge

  6. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

    Enlarge

  7. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

    Enlarge

  8. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162686-plasmid-map-sequence-id-321468

    Enlarge

  9. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

    Enlarge

  10. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Article
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

    Enlarge

  11. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

    Enlarge

  12. pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)

    Viral Prep
    #106176-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Promoter
    Syn
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-EF1a-iFlpV (AAV1)

    Viral Prep
    #140137-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-EF1a-iDreV (AAV1)

    Viral Prep
    #140136-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-Ef1a-DIO-PPO-Venus (AAV9)

    Viral Prep
    #139505-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Promoter
    EF1a
    Tags
    Venus (Cre-dependent)
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-EF1a-iCreV (AAV1)

    Viral Prep
    #140135-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-Ef1a-DIO-PPO-Venus (AAV8)

    Viral Prep
    #139505-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Promoter
    EF1a
    Tags
    Venus (Cre-dependent)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-EF1a-iFlpV (AAV PHP.eB)

    Viral Prep
    #140137-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-EF1a-iDreV (AAV PHP.eB)

    Viral Prep
    #140136-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2461 - 2480 of 2488 results